Papilloma virus and carcinoma

In addition to tobacco papilloma virus and carcinoma alcohol abuse, certain viruses have been associated with squamous cell carcinoma SCC of the head and neck, causing alterations in DNA. It has been demonstrated that the human papil­loma­virus HPV type 16, a subtype of the human pa­pil­loma­virus, is present in the oropharyngeal carcinomas of non-smokers patients inclusive.

HPV-infected cells express some viral proteins encoded by genes called E6 and E7, and can inactivate p53 protein and the retinoblastoma-type pro­tein RBP involved in the regulation of proliferation and cell death. Materials and method.

We present an immunohistochemical study conducted to identify significant tumour markers in tonsillar SCC.

Case Report

We present the sta­tis­tically significant correlations between the presence of immunohistochemical markers and studied local re­cur­rence, lymph node recurrence and risk of a second can­cer in the aerodigestive upper tract. The de­mon­stration of HPV in papilloma virus and carcinoma tumour tissue requires in situ hybridization or polymerase chain reaction PCR for the evidence of viral genome included into the host cell.

The practical implications of an etiologic role of HPV in head and neck cancer generally and in tonsillar SCC in particular remains in question and is in relate with prog­nosis, treatment and prevention. În afară de consumul de tutun şi abuzul de al­cool, anumite virusuri au fost asociate cu carcinomul cu celule scuamoase CCS al capului şi gâtului, cauzând al­te­rări la nivelul ADN-ului.

În anumite ţări papilloma virus and carcinoma venituri reduse din Asia şi Africa, prevalenţa HPV este foarte asemănătoare la femeile din toate  grupele de vârstă. Tipurile HPV 16 şi 18 au fost cele mai frecvente la scară mondială, HPV  16 fiind tipul cel mai întâlnit în toate regiunile. HPV 18 şi ale tipuri oncogene precum 31, 39, 51,  52, 56, 58 şi 59, au avut o prevalenţă similară şi au făcut parte din tipurile cele mai întâlnite după  HPV Femeile infectate cu un tip de HPV dat pot fi co- infectate sau infectate ulterior cu alte  tipuri care pot cauza leziuni cervicale. Nu s - a observat nicio tendinţă clară în funcţie de vârstă.

Este dovedit că virusul papiloma uman HPVtipul 16, este prezent la nivelul carcinoamelor orofaringiene inclusiv în cazul nefumătorilor. Celulele in­fec­ta­te cu HPV exprimă unele proteine virale codate de ge­ne­le denumite E6 şi E7 şi pot inactiva oxiuros en mujer embarazada p53 şi pro­tei­na de tip retinoblastom RBP implicate în reglarea pro­li­fe­ră­rii şi morţii celulare.

Materiale şi metodă. Pre­zen­tăm un stu­diu imunohistochimic realizat cu scopul de a identifica mar­keri tumorali semnificativi în CCS de amig­da­lă. Pre­zen­tăm co­re­la­ţiile semnificative statistic între prezenţa mar­ke­rilor imu­no­his­to­chimici şi recurenţa locală, recurenţa no­du­lilor limfatici şi ris­cul apariţiei unui al doilea cancer în trac­tul aerodigestiv su­pe­rior.

Punerea în evidenţă a HPV-ului în ţesutul tu­mo­ral amigdalian necesită hibridizare in situ şi reacţie de anemia 2 year old în lanţ PCR pentru punerea în evidenţă a genomului viral conţinut în celula-gazdă. Im­pli­caţiile practice ale unui rol etiologic al HPV-ului în can­ce­rele de cap şi gât, în general, şi în CCS de amigdală, în par­ti­cu­lar, reprezintă un subiect în dezbatere, fiind în relaţie cu prog­nos­ticul, tratamentul şi prevenţia acestor tipuri de can­cere.

Cuvinte cheie carcinomul cu celule scuamoase de amigdală CCS HPV markeri tumorali Introduction The tonsillar squamous cell carcinoma SCC is becoming a public health problem because of its rising incidence in the last 20 years, in contrast to the decreasing incidence of carcinomas in other subsites of head and neck associated to the reduced prevalence of smoking.

These tumours of oral cavity, oropha­rynx, larynx, hypopharynx and sinonasal region are linked by common characteristics, including a male predominant appearance in the 5th-6th decade of life, an important etiological link with tobacco, alcohol use or betel nut chewing, and a histopathological resemblance 1.

Data regarding the epidemiology papilloma virus and carcinoma that in Romania the oropharyngeal cancer represents 2. In France, during the last 30 years, the mortality in oral and oropharyngeal cancer increased by three times 1. As in cervical cancers, the oropharyngeal infection with HPV is a sexually transmitted disease which involves some particularities of sexual behaviour: a large number of vaginal sex partners, oral and anal sex. The recent increasing of OPSCC incidence may reflect the social changes regarding sexual behaviour in the modern world 6.

The anatomical sites preferred papilloma virus and carcinoma HPV in oropharynx are the tonsils and the tongue, because of the unique presence of transitional mucosa in oropharynx and particular in tonsillar tissue, cancer de orofaringe tratamento presents important histological similarities with the cervical mucosa.

Video CSID

Tonsillar epithelium invagination may favour virus capture and promote its access to basal cells the only papilloma virus and carcinoma cells in the epithelium. The tonsillar tissue could be a reservoir for HPV in the upper aero digestive tract. We had two premises for our study on tonsillar cancers. The second consists in the fact that mutagens such as tobacco, alcohol and HPV viral oncogenes E6 and E7 induce dysfunctions of two major mechanisms of cellular cycle, which involves the p53 and RBP tumoral suppressor genes 2.

  1. В таких случаях все роботы действуют одинаково, стремясь н самозащите.

  2. Human papillomavirus 52 positive squamous cell carcinoma of the conjunctiva
  3. Ovarian cancer abdominal discomfort
  4. Que es cancer biliar

Materials and method We made an immunohistochemical papilloma virus and carcinoma study between andaiming to identify any correlations between tumoral markers and the evolution and prognosis in tonsillar SCC.

Materials We studied 52 cases of patients diagnosed with tonsillar SCC. We had a first group Group I with 25 cases, where the positive diagnose was made by biopsy and these patients had radiotherapy as first curative papilloma virus and carcinoma of treatment.

We had a second group Group II with 27 cases, where the positive diagnose was made on surgical specimens and these patients had surgery as the first curative method of treatment. The two groups were similar regarding age and gender distribution. The dilutions and markers specifications are revealed in Table papilloma virus and carcinoma.

We also studied lymphocyte populations CD4, CD8, and populations of dendritic cells in tumour tissue. Table 1. The dilutions and markers specifications For the immunohistochemical identification of tumoral antigens we used the three-stadial hpv cancer uterino method Avidine-Biotine-Peroxidase ABPafter Hsu and colab.

Results The gender repartition of cases was: 47 male cases and 5 female cases. The age repartition of cases was: two cases between years old, 14 cases between years old, 21 cases between years old, 10 cases between years old, and five cases between years old. The correlation coefficient between papilloma virus and carcinoma two sets of data, corresponding to Group I and Group II, was 0. In both groups, we had 48 smoker patients, representing The patients who were both smokers and alcohol consumers represented We studied the tumoral markers on 52 cases of squamous cell carcinoma.

Thirty-eight cases papilloma virus and carcinoma well differentiated carcinoma and 14 cases were medium differentiated carcinoma. We present the results, that we considered immunohistochemically valid and statistically significant Table 2. Table 2. The distribution of tumoral markers in specimens of SCC studied We realised a correlation between the presence of the tumoral marker of a certain type positive and slowly positive results and the post-therapeutic evolution — local recurrence, nodal relapse, the occurrence of second cancers in upper aerodigestive upper ways and distance metastases.

We have had patients who had more than one recurrence in the same time. Our purpose was to identify the correlations between markers of evolution and prognosis in tonsillar SCC.

Throat Cancer and the Human Papilloma Virus

Our results indicate p53 protein and RBP protein as tumoral markers of unfavourable prognosis for post-therapeutic evolution in tonsillar SCC. For TGFa, we can make a correlation between its level in tumoral tissue and the risk of loco-regional relapse.

For the HPV identification in tumoral tissue, we used the identification of capsid p16 protein, so we cannot papilloma virus and carcinoma definitive conclusions referring at the presence or absence of HPV in the tumoral tissue for patients with tonsillar SCC. But we realised a correlation between the presence of HPV and the type of post-therapeutic evolution Figures Figure 1. The presence of RBP protein 48 positive and slowly positive cases was associated with local recurrence in 29 cases The presence of TGF protein 41 positive and slowly positive cases was associated with local recurrence in 18 cases The presence of HPV capsid protein 14 positive cases was associated with local recurrence in nine cases Figure papilloma virus and carcinoma.

Tumoral markers in evolution of tonsillar SCC result of our retrospective study From our data, we can certify as prognostic factors in tonsillar SCC: T stage, N stage, performing or not an elective type of clinical negative neck N0, type of neck dissection, the total dose of radiotherapy. We cannot make papilloma virus and carcinoma significant conclusions referring to the HPV presence in tumoral tissue mst papillomavirus symptomes tonsillar SCC and long-term prognosis.

Demonstrating the presence of HPV in tonsillar tumoral tissue imposes hybridisation in situ or polymerase chain reaction PCR.

Discussion Slaughter et al. They explained the greater risk for multiple primary cancers 8.

  • Jump to navigation Jump to search Cancerul de col uterin este un neoplasm malign al colului uterin sau cervixului cum mai este numit.
  • Infecţia cu virusul HPV (Human papilloma virus)
  • Hpv bulasma yollar?
  • Gel aloe vera pentru detoxifiere
  • El papiloma es benigno o maligno
  • Top 5 virusi

Tobacco and alcohol abuse increase the risk for a second cancer development in patients with oropharyngeal SCC. Tobacco and alcohol abuse are associated with mutations of the p53 protein in patients papilloma virus and carcinoma OFSCC, being important factors in the molecular progression through carcinogenesis 9.

Many clinical studies searched for the p53 protein mutations on surgical specimens from patients with OFSCC. The papilloma virus and carcinoma with surgical positive edges for p53 protein mutations have a higher risk of local relapse P53 protein mutations are involved in the loco-regional failure at OFSCC tonsillar with curative radiotherapy The HPV 16 DNA was identified only in primary tumour cells and in their metastases in similar manner with cervical cancer 3.

The presence of HPV 16 genome was revealed by polymerase chain reaction PCR or the method of hybridisation in situ, which certified the presence of viral genome included in host cell genome It is necessary to make a study on HPV tonsillar infection in non-smokers and non-alcoholic consumers. Our method for HPV identification was immunohistochemistry for p16 protein, which is a specific capsid protein of HPV 16 type, so we cannot certify the presence of HPV genome in all specimens studied.

Human papillomavirus 52 positive squamous cell carcinoma of the conjunctiva

We were in the situation of the unavailability of the in situ hybridization kits or polymerase chain reaction for HPV 16 type during the study. We have to mention the high cost for identifying viral markers. Clinical stadialization represents the primary guide to choose the therapeutic modality, but it is a limited guide.

We expected the genetic analysis to be the method of future, meaning the identification of markers for prevention, therapy and good prognosis. Recent studies showed an inverse correlation between the presence of HPV and p53 protein mutations. The HPV-positive tumors have genetic alterations associated with a better answer to chemotherapy and with papilloma virus and carcinoma improved radio-sensitivity.

The immune response of the patient is better, because of the immune stimulation realised papilloma virus and carcinoma viral antigens. The younger age with less comorbidities may contribute to a better prognosis Prophylactic vaccination is not efficient in already diagnosed infections and in malignant lesions, so it is necessary to study the efficiency of therapeutic HPV vaccination in the treatment of HPV-associated cancer 1.

Cancer de col uterin

We found in lite­rature premises for the therapeutic vaccination in HPV-induced cancers, where this type of vaccine induced a cytolytic immune response in cells which express it 5. It is necessary to study the carriage of HPV in apparently normal tonsillar tissue, to have screening programmes and to select the patients at risk for OFSCC Conclusions The prognosis in tonsillar SCC is mediocre, due to local recurrence, nodal relapse, the occurrence of second cancers in upper aerodigestive upper ways 17 and to distance metastases.

From our data, we can certify as prognostic factors in tonsillar SCC: T stage, N stage, performing or not an elective type of clinic negative neck N0, type of neck dissection and total dose of radiotherapy. We cannot draw significant conclusions referring to the HPV papilloma virus and carcinoma in tumoral tissue in tonsillar SCC and regarding the prognosis signification of the HPV presence in the tumoral tissue.

The presence of HPV 16 can be considered a positive prognostic factor for disease-free survival and for healing, but the use as a predictive marker has not yet been proven. The best treatment against cancer is prevention, especially in malignancies where the main pathogen agent is known, and we are talking here about smoking, alcohol consume and a papilloma virus and carcinoma sexual behaviour.

Ethical approval: All authors hereby declare that all experiments have been examined and approved by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in the Declaration of Helsinki. She has done an accurate immunohistochemical examination of paraffin-embedded specimens.

Conflict of interests: The authors declare no conflict of interests. Acta Otorhinolaryngol Italica. Human papillomavirus infection as a risk factor for squamous-cell carcinoma of the head and neck.

Department of Ophthalmology, Grigore T. E-mail: moc. We report the detection of HPV 52 in a hookworm taken from a year-old patient with squamous cell carcinoma of the conjunctiva of the left eye. The method used for the detection of HPV was real time polymerase chain reaction. The evolution was favorable after surgical removal of the tumor and the patient was explained that long-term follow-up is essential to avoid recurrence.

New England Journal of Medicine. New insights into human papillomavirus-associated head and neck squamous cell carcinoma. Tonsillar and other upper aerodigestive tract cancers among cervical cancer patients and their husbands. European Journal of Cancer Prevention. Successful therapeutic lentiviral vector defective integrase vaccination with human papillomavirus E7 protein Expressing nononcogenic. Int J Cancer.

Frequent p53 mutations in head and neck cancer. Cancer Res. Quantification of surgival margin shrinkage in papilloma virus and carcinoma oral cavity. Head and Neck.

Infecţia cu virusul HPV (Human papilloma virus)

Journal of National Cancer Institute. Human papillomavirus and DNA ploidy in tonsillar cancer — correlation to prognosis. Anticancer Researches. Altered antigen expression predicts outcome in squamous cell carcinoma of the head and neck. Human papillomavirus positivity predicts favourable outcome for squamous carcinoma of the tonsil.

Cancer de col uterin - Wikipedia

International Journal of Cancer. Detection of human papillomavirus type 16 in carcinoma of the palatine tonsil. Journal of Clinical Pathology. Second neoplasm in patients with head and neck cancer. Head Neck.

Ațiputeafiinteresat