Rotar, Florin V. Interleukin 1β is a proinflammatory cytokine that plays a critical role in chronic inflammation and carcinogenesis. Material and method.
A case-control study was realized. The cases were represented by patients diagnosed with cervical intraepithelial papilloma virus stadio 16, positive at HPV HR testing, while controls, negative for intraepithelial neoplasia and negative at HPV HR testing, were included in the control group.
- Corelaţii clinice şi paraclinice în managementul neoplaziei intraepiteliale cervicale
- Cervical precancer | Cabinet ginecologie
- Variantele genice ale Interleukinei 1β C>T şi neoplazia intraepitelială cervicală
Chi-square statistics had a value of 0. Odds ration had values of 1.
Designul studiului a fost de tip caz-control. Lotul de studiu a fost reprezentat de de paciente diagnosticate cu neoplazie cervicală intraepitelială şi pozitive la testarea HPV, iar lotul de control a fost reprezentat de paciente fără leziuni cervicale şi negative la testarea HPV. The mechanisms involved in this transition are extremely complex and only partially understood, immune signal molecules, chemokines, interleukins and the cervical cancer bethesda classification involved in the control of the cellular cycle being involved 1.
Genetic particularities of these molecules could explain why fortunately only a small number of HPV Human Papilloma Virus infected women will develop cervical cancer. Interleukin 1b together with interleukin-1a and the antagonist of interleukin 1 belong to cervical cancer bethesda classification interleukin 1 cytokine family, hpv warzen auf der zunge on a kb segment on chromosome 2.
Interleukin 1b is synthesized mainly by macrophages, but also by other cells of the immune system, with important roles in both acute and chronic inflammatory response 2.
The fact that chronic HPV infection is a mandatory step in cervical carcinogenesis is well known 3. Previously published researches confirmed the contribution of interleukin 1b in the neoplastic process by influencing tumor growth and angiogenesis 4respectively in the induction of chemo resistance 5.
While performing a search throughout MEDLINE databases we have encountered articles regarding the association between this polymorphism and human pathologies 8.
Но это были лишь разорванные обрубки огромных дорог: оживлявшее их удивительное вещество застыло неподвижно.
The presence of this polymorphism was found to be significantly associated with gastroduodenal ulcer 9neoplasia 10pulmonary 11 and pancreatic cancer This particular genetic polymorphism was proved to be related to gynecological and obstetrical conditions: high risk of preterm labor 16spontaneous recurrent abortions 17,18bacterial vaginosis 19polycystic ovary syndrome 20 and uterine leiomyoma The relationship between this genetic polymorphism and cervical cancer was previously cervical cancer bethesda classification in two small studies cervical cancer bethesda classification an Indian and respectively Korean population but not in Caucasians 22, One hundred and eleven patients with negatives results for intraepithelial lesion with negative HPV HR tests were included in the control group.
Cervical cancer bethesda classification patients were recruited from the corpus of patients hospitalized or only consulted on an ambulatory base during this period at the First Clinic of Obstetrics and Gynecology, Emergency County Hospital Cluj-Napoca, Romania, willing to participate at this study. The cervical cancer bethesda classification was approved by the ethical committees and the written consent was obtained for each patient Biological probes collection A cervical cancer bethesda classification examination was performed for each patient.
A cervical cytology was obtained.
Этому мирному краю тоже предстояло перемениться. Олвину частенько приходило в голову -- правильно ли он поступил, открыв в своем безжалостном стремлении удовлетворить собственное любопытство древний путь, связывающий обе культуры. Но конечно же лучше было, чтобы Лиз узнал правду,-- ведь и он, как и Диаспар, почивал на своих собственных опасениях и совершенно беспочвенных мифах.
Иногда Олвин задумывался и над тем, какие же черты приобретет новое общество.
Cervical cytology results were formulated according to Bethesda nomenclature Colposcopic examination was performed for each patient.
After the gynecological examination a blood sample was obtained using a 2 ml blood collection tube.
The blood samples were immediately stored at cervical cancer bethesda classification. The analysis of cervical samples The cervical cytology was examined by a cytologist after a preliminary Papanicolau staining in the laboratory of the above mentioned hospital. HPV HR testing was performed using an immunohistochemistry-based kit. DNA extraction. Genetic analysis The genomic DNA was extracted from peripheral leukocytes contained in µl of whole blood using a commercial kit Wizzard Genomic DNA Purification Kit, Promega® according to the producer instructions.
The extracted DNA was stored in a freezer at °C.
This is achieved by the excision or ablation of the squamous-cylindrical area up to the healthy tissue. In the case of persistence of high-grade lesions after repeated excision and if the patient does not want to preserve fertility, hysterectomy may be indicated. This is a surgical procedure that can be indicated for benign gynaecological pathology, preinvasive cervical neoplasia and stage IA1 of cervical cancer.
Briefly, the PCR reaction was done in 25 µl, total volume containing: Free nuclease water was added in order to reach the total volume.
The PCR protocol is shown in Table 2.
Table 1. Statistical analysis The quantitative continuous variables were expressed using centrality and dispersion parameters. Comparison of the distribution of genotypes on cases and controls was done using chi-square test.
Variantele genice ale Interleukinei 1β -511 C>T şi neoplazia intraepitelială cervicală
The differences were considered significant if p-value was lower than 0. CatROM program 28 was used in order to calculate the statistical parameters associated to the 2×2 contingency table for investigation of genetic polymorphisms as risk factor for cervical cancer. Results One hundred and twenty eight cases and one hundred and eleven controls were enrolled in this study. Each case had a diagnosis of cervical intraepithelial neoplasia based on cervical cytology.
In patients with intraepithelial neoplasia, HSIL is not the most frequent pathology encountered 31 ; it represents the consequence of the study design. Figure 1. Categories of cervical intraepithelial neoplasia - case group Genotypes distribution is presented in Table 3.
Table 3. The frequencies of IL1 β genotypes for case and control groups Genotypes distribution for each type of cervical intraepithelial neoplasia is presented in Figure 2. Table 4.
Allelic frequency for case groups and p-value associated to Z test when the cervical cancer bethesda classification on case and control groups were compared Table 5. The common allele - C - was the most frequently encountered in both groups and in all categories of cases. The results obtained in analysis on control group are as follow: C: